mouse anti myo7a  (Santa Cruz Biotechnology)

Journal: bioRxiv

Article Title: Cell-specific delivery of GJB2 restores auditory function in mouse models of DFNB1 deafness and mediates appropriate expression in NHP cochlea

doi: 10.1101/2024.12.24.630240

Figure Lengend Snippet: a. Vehicle-injected cochlea. Anti-SOX2 labeling (magenta), anti-MYO7A labeling (yellow) and DAPI-labeled nuclei (blue) were detected. b. AAV- GRE-hsGJB2.HA-injected cochlea. Robust anti-HA labeling (red) was detected in the same locations as endogenous GJB2, analysis of cochlear turns showed no gradient in GJB2.HA expression between apex, middle and base. Scale bars = 1 mm.

Article Snippet: For immunofluorescence labeling, the following primary antibodies and secondary antibodies were used: mouse anti-MYO7A antibody (1:50) (#sc-74516, Santa Cruz), rabbit anti-HA C29F4 antibody (1:200) (#3724, Cell Signaling), rabbit anti-GJB2 antibody (1:50) (#71-0500, Fisher), goat anti-SOX2 antibody (1:50) (#AF2018, R&D Systems), rabbit anti-IBA1 antibody (1:200) (#019-19741, Wako Chemicals), donkey anti-rabbit IgG secondary antibody conjugated to Alexa Fluor 594 (1:200) (Invitrogen), donkey anti-goat IgG conjugated to Alexa Fluor 647 (1:200) (Invitrogen), donkey anti-mouse IgG conjugated to Alexa Fluor 488 (1:200) (Invitrogen), donkey anti-mouse IgG conjugated to Alexa Fluor 594 (1:200) (Invitrogen), and donkey anti-rabbit IgG conjugated to Alexa Fluor 488 (1:200) (Invitrogen).

Techniques: Injection, Labeling, Expressing